3+ day, 18+ hour ago   (128+ words) Table 1: Summary of contamination types in NGS data, with characteristic data signatures and recommended analytical detection tools. Reads with unexpected or mismatched index combinations Unique dual index audit, per-library read-count review A systematic approach for evaluating a sample flagged for…...

3+ day, 18+ hour ago   (156+ words) Mapped 5' start position Mapped start position and UMI sequence Distinguishes independent molecules at the same position Appropriate for WGS germline variant calling Yes, with added overhead Appropriate for deep targeted panel sequencing No; removes genuine fragments Yes, via consensus calling…...

5+ day, 17+ hour ago   (222+ words) Table 1: Comparison of poly-A selection and ribosomal RNA depletion for RNA library preparation across key decision parameters. Limited; many lncRNAs partially or fully excluded Performance on degraded RNA Strong 3' end bias Relative sequencing depth needed Figure 1: A comparison of poly-A…...

6+ day, 23+ hour ago   (239+ words) The human body and its gut microbiome produce thousands of small molecules that shape how the body functions—influencing immunity, metabolism, and more. Identifying what those molecules actually are has been one of the biomedical sciences’ most persistent bottlenecks. More…...

1+ week, 3+ day ago   (457+ words) Evolution has created an astonishing amount of diversity, shaping much of our understanding of biology. But this diversity, which took billions of years to emerge, barely scratches the surface of what could have existed given the trillions of possible DNA…...

1+ week, 5+ day ago   (720+ words) Influenza continues to cause major illness each year in the United States, leading to more than 35,000 deaths annually. Children, older adults, pregnant individuals, people with weakened immune systems, and those with chronic conditions such as heart disease, metabolic disorders, and…...

1+ week, 6+ day ago   (388+ words) Nanopore sequencing read length is constrained by the length of the input DNA fragment, not the chemistry. In principle, the pore will continue reading for as long as the molecule threading through it remains intact. Practical limits arise from sample…...

2+ week, 5+ day ago   (339+ words) Kidney disease is silent in its early stages, progressing without symptoms until the disease is advanced. Researchers in the McKelvey School of Engineering at Washington University in St. Louis, in collaboration with researchers at Texas A&M University and Washington…...

2+ week, 4+ day ago   (1204+ words) Everything started back in 1982, when the US Food and Drug Administration (FDA) approved the first recombinant insulin, marking the birth of the modern biologics industry. Previously, insulin and other biologics were made using animal cells that naturally produced biomolecules, a…...

2+ week, 5+ day ago   (449+ words) Scientists can engineer living cells to perform useful tasks, from producing medicines and sustainable chemicals to detecting disease. Unlike traditional engineered systems, living cells are constantly changing as they grow, making their behavior difficult to predict and control. Dr. Chelsea…...